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fa disassembly assays  (Oxford Instruments)


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    Structured Review

    Oxford Instruments fa disassembly assays
    ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) <t>Disassembly</t> T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.
    Fa Disassembly Assays, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44064 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa+disassembly+assays/Imaris/pmc11957695-201-5-9
    Average 99 stars, based on 44064 article reviews
    fa disassembly assays - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression"

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI189197

    ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.
    Figure Legend Snippet: ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.

    Techniques Used: Staining, Transfection, Immunoprecipitation, Isolation

    Related Articles

    Staining:

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study ( ).

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study (51).

    Transfection:

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study ( ).

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study (51).

    Immunoprecipitation:

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study ( ).

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study (51).

    Isolation:

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study ( ).

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression
    Article Snippet: cell imaging, the raw images were processed/analyzed in Imaris 9.6 (Bitplane software, Oxford instruments) with MATLAB XTensions and Fiji/ImageJ. .. Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode. .. Disassembly rate constants and half-life values were calculated according to a previous study (51).



    Similar Products

    fa disassembly assays  (Oxford Instruments)
    99
    Oxford Instruments fa disassembly assays
    ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) <t>Disassembly</t> T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.
    Fa Disassembly Assays, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fa+disassembly+assays/Imaris/pmc11957695-201-5-9
    Average 99 stars, based on 1 article reviews
    fa disassembly assays - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression

    doi: 10.1172/JCI189197

    Figure Lengend Snippet: ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.

    Article Snippet: Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode.

    Techniques: Staining, Transfection, Immunoprecipitation, Isolation